New Nile blue derivatives as NIR fluorescent probes and antifungal agents

The synthesis of four new Nile Blue derivatives with hydrogen, propyl and/or aminopropyl groups as substituents of the amines of 5- and 9-positions is described. Photophysical properties were evaluated in acidified ethanol and aqueous solution at physiological pH. Antifungal activity is also studied through the obtention of MIC values.Thanks are due to Fundação para a Ciência e Tecnologia (FCT) and FEDER (European Fund for Regional Development)-COMPETE-QRENEU for financial support through the Chemistry Research Centre of the University of Minho (Ref. UID/QUI/00686/2013 and UID/QUI/0686/2016), CBMA (PEst OE/BIA/UI4050/2014) and a PhD grant to J.C.F. (SFRH/BD/133207/2017). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project No 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC)

N-(5-Amino-9H-benzo[a]phenoxazin-9-ylidene)propan-1-aminium chlorides as antifungal agents and NIR fluorescence probes

The search for benzo[a]phenoxazines, Nile Blue derivatives, with high antifungal activity and cell labelling capacity based on our previously published works in this type of compounds, led us to the design of compounds with specific substituents in the polycyclic system. Thus, in the present work, four new benzo[a]phenoxazinium chlorides, possessing at the 5-position amino or (3-aminopropyl) amino groups and at the 9-position propylamino or dipropylamino groups, were synthesized. Another analogue, with (3-aminopropyl) amino group at 5-position, ethyl amino group at 9-position and a methyl group at 10-position of the polycyclic system was also synthesized for comparison in the studies performed. Fundamental photophysics (absorption and fluorescent emission) was carried out in absolute ethanol, water, and other aqueous solutions of different pH values, relevant for the potential biological applications of these compounds. The antiproliferative activity of the synthesized benzo[a]phenoxazinium chlorides was determined using Saccharomyces cerevisiae PYCC 4072 and the microdilution method described for antifungal susceptibility tests in yeast. All compounds revealed antifungal activity, being the most active the one possessing an amino group at 5-position and an aminopropyl group at 9-position. The potential as fluorescent probes were evaluated by fluorescence microscopy, using S. cerevisae as a model system of eukaryotic cells, and it was found that the benzo[a]phenoxazinium chlorides stained the cells with preferential accumulation that seems to appear at the vacuolar membrane and/or the perinuclear membrane of the endoplasmatic reticulum.Thanks are due to Fundação para a Ciência e Tecnologia (FCT) and FEDER (European Fund for Regional Development)-COMPETE-QRENEU for financial support through the research centres CQ/UM (UID/QUI/0686/2019 and UIDB/00686/2020) and CBMA (PEst-OE/BIA/UI4050/2019 and UID/BIA/04050/2020), as well as a PhD grant to J. C. F. (SFRH/BD/133207/2017). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network (PTNMR) and is partially supported by Infrastructure Project No. 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC)

Ground motion simulation for dynamic structural analysis: pros and cons

In many seismic codes the use of simulated ground motions for dynamic structural analysis is already considered. However, from the structural engineer point of view, the use of such simulated accelerograms is not very attractive, mainly because more seismological knowledge background is needed. For this reason, a user friendly freeware computer program named SIMULSIS was developed to help structural engineers to generate simulated accelerograms. A new finite-fault stochastic method was developed for ground motion simulation and implemented in the program together with an equivalent linear model that accounts for superficial soil dynamic amplification. The capabilities of the program and the precision of results produced are illustrated by the simulations carried out for two recorded earthquakes. Moreover, some suggestions related to the use of the developed computer program not only for seismic structural analysis purposes but also in the future generation of hazard maps are presented

Comparative analysis of Algarve`s seismic hazard

In this paper we compared the seismic hazard of Algarve obtained with two different models: a point-source model (proposed by McGuire) and a fault-rupture model (proposed by Der Kiureghian and Ang)

A novel control architecture based on behavior trees for an omni-directional mobile robot

Robotic systems are increasingly present in dynamic environments. This paper proposes a hierarchical control structure wherein a behavior tree (BT) is used to improve the flexibility and adaptability of an omni-directional mobile robot for point stabilization. Flexibility and adaptability are crucial at each level of the sense–plan–act loop to implement robust and effective robotic solutions in dynamic environments. The proposed BT combines high-level decision making and continuous execution monitoring while applying non-linear model predictive control (NMPC) for the point stabilization of an omni-directional mobile robot. The proposed control architecture can guide the mobile robot to any configuration within the workspace while satisfying state constraints (e.g., obstacle avoidance) and input constraints (e.g., motor limits). The effectiveness of the controller was validated through a set of realistic simulation scenarios and experiments in a real environment, where an industrial omni-directional mobile robot performed a point stabilization task with obstacle avoidance in a workspace.This work was financed by national funds from the FCT (Foundation for Science and Technology), I.P., through IDMEC under LAETA, project UIDB\50022\2020. The work of Rodrigo Bernardo was supported by the PhD Scholarship BD\6841\2020 from the FCT. This work indirectly received funding from the European Union’s Horizon 2020 programme under StandICT.eu 2026 (Grant Agreement No. 101091933).info:eu-repo/semantics/publishedVersio

Novel benzo[α]phenoxazinium chlorides functionalized with sulfonamide groups as NIR fluorescent probes for vacuole, endoplasmic reticulum, and plasma membrane staining

The demand for new fluorophores for different biological target imaging is increasing. Benzo[a]phenoxazine derivatives are fluorochromophores that show promising optical properties for bioimaging, namely fluorescent emission at the NIR of the visible region, where biological samples have minimal fluorescence emission. In this study, six new benzo[a]phenoxazinium chlorides possessing sulfonamide groups at 5-amino-positions were synthesized and their optical and biological properties were tested. Compared with previous probes evaluated using fluorescence microscopy, using different S. cerevisiae strains, these probes, with sulfonamide groups, stained the vacuole membrane and/or the perinuclear membrane of the endoplasmic reticulum with great specificity, with some fluorochromophores capable of even staining the plasma membrane. Thus, the addition of a sulfonamide group to the benzo[a]phenoxazinium core increases their specificity and attributes for the fluorescent labeling of cell applications and fractions, highlighting them as quite valid alternatives to commercially available dyes.FCT (Fundação para a Ciência e Tecnologia, Portugal) and FEDER (European Fund for Regional Development)-COMPETEQREN-EU for financial support to the research centers CQ/UM (UID/QUI/00686/2021), and CBMA (Ref. UIDB/04050/2020), as well as a PhD grant to J. C. Ferreira (SFRH/BD/133207/2017 and COVID/BD/151978/2021). The NMR spectrometer Bruker Avance III 400 (part of the National NMR Network) was financed by FCT and FEDER

Avaliação, educação e comunicação do risco sísmico no contexto das escolas do Algarve

Tem sido observado, um pouco por todo o mundo, que as populações não têm consciência do risco sísmico dos edifícios onde habitam, e que possuem espectativas irrealistas sobre o comportamento dessas construções. A perceção generalizada é que as habitações irão ficar intactas após um sismo moderado ou destruidor, desde que estas tenham sido corretamente projetadas e construídas, de acordo com as normas vigentes. Contudo, os edifícios somente são projetados para garantir a salvaguarda da vida humana, e não para a salvaguarda do património em si, podendo até não ser economicamente viável a sua reparação. Além disso, um sismo moderado poderá causar danos moderados ou severos, principalmente a nível não-estrutural, afetando assim o funcionamento das instalações por tempo indeterminado. Por outro lado, é importante promover a educação e informação das populações para contribuir para o aumento da resiliência das sociedades a estes fenómenos. Neste contexto, foi desenvolvida uma plataforma informática no âmbito do projeto de investigação PERSISTAH (Projetos de Escolas Resilientes aos SISmos no Território do Algarve e de Huelva), que possibilita a realização de avaliações de segurança sísmica dos edifícios das escolas do primeiro ciclo do ensino básico existentes no Algarve, recorrendo a modernas técnicas de análise estrutural. Usando esta ferramenta, é possível verificar qual o grau de dano provável de um edifício de uma escola, quer seja para um qualquer cenário de ocorrência de um sismo, ou simplesmente no contexto da verificação dos níveis de segurança estabelecidos nas normas NP EN 1998-1:2010 e NP EN 1998-3:2017. Estão a ser criados documentos direcionados para técnicos da área da construção, para aprendizagem das referidas metodologias, assim como relativas ao uso de técnicas de reabilitação sísmica. Em paralelo, também foram desenvolvidos instrumentos para aumentar a resiliência sísmica da população estudantil, designadamente o Projeto Educativo “Porquê que o chão se move?”, com atividades e material pedagógico para a educação e comunicação do risco sísmico, e o “Guia Prático da Escola Resiliente aos Sismos”, que também visam a implementação de medidas de redução do risco sísmico não-estrutural.info:eu-repo/semantics/publishedVersio

Adaptation and convergence in circadian‐related genes in Iberian freshwater fish

The circadian clock is a biological timing system that improves the ability of organisms to deal with environmental fluctuations. At the molecular level it consists of a network of transcription-translation feedback loops, involving genes that activate (bmal and clock - positive loop) and repress expression (cryptochrome (cry) and period (per) - negative loop). This is regulated by daily alternations of light but can also be affected by temperature. Fish, as ectothermic, depend on the environmental temperature and thus are good models to study its integration within the circadian system. Here, we studied the molecular evolution of circadian genes in four Squalius freshwater fish species, distributed across Western Iberian rivers affected by two climatic types with different environmental conditions (e.g., light and temperature). S. carolitertii and S. pyrenaicus inhabit the colder northern region under Atlantic climate type, while S. torgalensis, S. aradensis and some populations of S. pyrenaicus inhabit the warmer southern region affected by summer droughts, under Mediterranean climate type.info:eu-repo/semantics/publishedVersio

From chickpea and lupin as dairy alternatives

Funding text This work was supported by the FCT Project PTDC/BAA-AGR/28370/2017: “Bebida de proteína vegetal a partir de leguminosas europeias com potencial bioativo”; and also through the research unit UID/AGR/04129/2020 – LEAF.Consumption of plant based products as dairy alternatives is increasing steeply. This diet transition can only be achieved if these products keep the nutritional value and meet consumer's sensory acceptance. This work aimed to evaluate the decrease of the “beany” flavour and of raffinose, stachyose and verbascose contents in EU pulse beveragés production, and also the best lactic fermentation conditions of the beverages, towards chickpea- and lupin-based yoghurts, with rheology properties similar to the commercial soy yoghurts. The reduction of “beany” volatile compounds of chickpea and lupin beverages during processing was confirmed through GC–MS analysis. Soaking and cooking processes were effective in removing flatulence sugars with almost 48% loss from the initial content in lupin beverage. The fermentation conditions at 40 °C, 12 h and 2% (w/v) of starter concentration evidenced the best viscoelastic structure and flow properties. The lupin yoghurt-type showed a similar gel structure to commercial soy yoghurt. © 2022publishersversionpublishe